Although researchers nonetheless have no idea precisely what triggers the uncoating of VP7, they do know that it appears to happen when the virus senses a lowered focus of calcium in its atmosphere. To get a closer take a look at how antibodies latch onto VP7 and neutralize the virus, Harrison and his colleagues used x-ray crystallography to examine the molecular architecture of VP7 within the grasp of a fragment of the antibody. X-ray crystallography is a robust software for “seeing” the orientation of atoms and the distances separating them throughout the molecules. Before Harrison’s group might use x-ray crystallography, however, they first needed to crystallize VP7 in complex with the antibody fragment. Only after that step was completed, could they transfer on to bombarding these crystallized proteins with x-rays. Computers helped seize the diffraction patterns that emerged as the x-rays scattered from the crystal lattice. By rotating the crystallized protein complexes by multiple exposures, the researchers might report enough knowledge to calculate three-dimensional models, which exposed the underlying architecture of VP7 and the antibody fragment.

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